Mass Spectrometry analysis-based Proteomics
Byung-Gyu Kim, Ph.D.
Mass Spectrometry analysis-based Proteomics / Metabolomics research
UNIST-Gil 50, Ulsan, 689-798 (44919), Bldg 103, Room 406, Republic of Korea
•Director General for International Cooperation, The Korean Human Proteome Organization (KHUPO)
•Finance Chairman, LOC, 2023 HUPO World congress (September 17-21, Busan, Korea, http://www.hupo2023.org/))
•Bone-muscle aging-related 4D Nucleome dynamics (School of Dentistry, SNU, director, Hyun-Mo Ryoo), 2021-2023
•Technology Transfer & consulting (MS-analysis for antibody), BIONOTE (SD biosensor), 2022-2023
Since Nov. of 2016, he joined to Center for Genomic Integrity (Institute for Basic Science, UNIST, Ulsan) as a Principal researcher and has investigated for MS-based proteomics (Orbitrap Fusion Lumos system) / metabolomics analysis for understanding about molecular mechanisms of DNA damage & repair system in organisms. He was also selected as an Early Career Researcher (ECR) of Rep. Korea in the 2018 Human Proteome Organization (HUPO). He has developed chemoproteomics with SILAC-CETSA approach to obtain On-target / Off-target profiles of interesting new molecule entities (NMEs) and investigated drug targets for various types of indications.
Orbitrap Fusion Lumos [Thermo Fisher Scientific ]
The Orbitrap Fusion Lumos Tribrid Mass Spectrometer is designed to expand performance in advanced proteomics, biopharma and metabolomics applications, including quantitation using isobaric tags, low level PTM analysis, data independent acquisition (DIA), and top down proteomics. The new instrument features enhanced sensitivity resulting in improved analyte detection, characterization and quantitation, enabling scientists to perform more comprehensive sample analyses faster and with better accuracy than ever before. The system is the research-leading high performance mass spectrometer with enhanced sensitivity facilitated by a new High Capacity Transfer Tube, Electrodynamic Ion Funnel, Advanced Quadrupole Technology, Advanced Vacuum Technology, and ETD HD.
Proteomics based DNA integrity research
• Affinity purification (AP)-MS based DNA integrity regulators interactome profiling
• Post-translational modification (PTM) identification of DNA integrity regulators
• Monitoring protein dynamics at replication fork with SILAC-iPOND analysis
• DNA integrity linked drug target identification
• Differential expressed protein (DEP) profiling